LOCUS       sequence-112176-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pUK
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..43)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        156..395
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
       61 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      121 cgtcgaggaa aactcttcaa tagttttgcc agcggaattc cacttgcaat tacataaaaa
      181 attccggcgg tttttcgcgt gtgactcaat gtcgaaatac ctgcctaatg aacatgaaca
      241 tcgcccaaat gtatttgaag acccgctggg agaagttcaa gatatataag taacaagcag
      301 ccaatagtat aaaaaaaaat ctgagtttat tacctttcct ggaatttcag tgaaaaactg
      361 ctaattatag agagatatca cagagttact cactaatgac taacgaaaag gtctggatag
      421 agaagttgga taatccaact ctttcagtgt taccacatga ctttttacgc ccacaacaag
      481 aaccttatac gaaacaagct acatattcgt tacagctacc tcagctcgat gtgcctcatg
      541 atagtttttc taacaaatac gctgtcgctt tgagtgtatg ggctgcattg atatatagag
      601 taaccggtga cgatgatatt gttctttata ttgcgaataa caaaatctta agattcaata
      661 ttcaaccaac gtggtcattt aatgagctgt attctacaat taacaatgag ttgaacaagc
      721 tcaattctat tgaggccaat ttttcctttg acgagctagc tgaaaaaatt caaagttgcc
      781 aagatctgga aaggacccct cagttgttcc gtttggcctt tttggaaaac caagatttca
      841 aattagacga gttcaagcat catttagtgg actttgcttt gaatttggat accagtaata
      901 atgcgcatgt tttgaactta atttataaca gcttactgta ttcgaatgaa agagtaacca
      961 ttgttgcgga ccaatttact caatatttga ctgctgcgct aagcgatcca tccaattgca
     1021 taactaaaat ctctctgatc accgcatcat ccaaggatag tttacctgat ccaactaaga
     1081 acttgggctg gtgcga
//
