LOCUS       sequence-112215-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK081
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    136..639
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     terminator      complement(661..695)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc agagtatcac gtgctataaa aataattata atttaaattt tttaatataa
      181 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
      241 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
      301 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaaa accacacacg
      361 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
      421 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
      481 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
      541 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
      601 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcccgatga gaccagacca
      661 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      721 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      781 ccactcat
//
