LOCUS       sequence-112220-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pUK
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(255..1058)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt
      181 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
      241 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
      301 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
      361 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
      421 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
      481 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
      541 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
      601 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
      661 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
      721 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
      781 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
      841 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
      901 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
      961 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     1021 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tt
//
