LOCUS       sequence-112225-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS, tagBFP and a KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP-KRAB
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..55)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(62..82)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(95..121)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(101..127)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgctgctgcc gttgctccca ctaccaatgc cgtccacttt ccgctttttc ttaggatctt
       61 ccaccttgcg ttttttcttg ggggagccgc tgcccaggct ggcataatcg ggcacgtcat
      121 agggataggc gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga
      181 tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt
      241 cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc cagattggtc agggtaaaca
      301 ggtggatgat attctcggcc tgctctctga taggcttgtc tctgtgcttg ttgtaggcgc
      361 tcagcacctt gtccagatta gcgtcggcca ggatcactct cttggagaac tcgctgatct
      421 gctcgatgat ctcgtccagg tagtgtttgt gctgttccac aaacagctgt ttctgctcat
      481 tatcctcggg ggagcccttc agcttctcat agtggctggc caggtacagg aagttcacat
      541 atttggaggg cagggccagt tcgtttccct tctgcagttc gccggcagag gccagcattc
      601 tcttccggcc gttttccagc tcgaacaggg agtacttagg cagcttgatg atcaggtcct
      661 ttttcacttc tttgtagccc ttggcttcca gaaagtcgat gggattcttc tcgaa
//
