LOCUS       sequence-112228-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes URA3 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pUM
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(316..1119)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataggat
      181 ccgcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
      241 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
      301 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
      361 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
      421 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc
      481 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc
      541 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct
      601 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
      661 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt
      721 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc
      781 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa
      841 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac
      901 tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
      961 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc
     1021 acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg
     1081 atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca
     1141 ggtttttgtt ctgtgcagtt gggtta
//
