LOCUS       sequence-112239-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-RhoA-Q63L
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(36..428,429..431,432..617))
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     primer_bind     complement(666..687)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actatagaat agggccctct agatgcatgc tcgagtcaca agacaaggca accagatttt
       61 ttcttcccac gtctagcttg cagagcagct ctcgtagcca tttcaaaaac ctctctcact
      121 ccatctttgg tctttgctga acactccatg tacccaaaag cgccaatcct gtttgccata
      181 tctctgcctt cttcaggttt caccggctcc tgcttcatct tggctagctc ccgccttgtg
      241 tgctcatcat tccgaagatc cttcttattc ccaaccagga tgatgggcac gttgggacag
      301 aaatgcttga cttctggggt ccacttttct gggatgtttt ctaaactatc agggctgtcg
      361 atggaaaaac acatcagtat aacatcggta tctgggtagg agaggggcct caggcgatca
      421 taatcttcca gcccagctgt gtcccacaaa gccaactcta cctgctttcc atccacctcg
      481 atatctgcca catagttctc aaacactgtg ggcacataca cctctgggaa ctggtccttg
      541 ctgaagacta tgagcaagca tgtctttcca caggctccat caccaacaat caccagtttc
      601 ttccggatgg cagccatgaa ttccttgtac agctcgtcca tgccgagagt gatcccggcg
      661 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      721 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      781 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      841 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      901 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      961 ttcagctcga tgcggttcac
//
