LOCUS       sequence-112240-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-RhoA-Q63L
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(9..194,195..197,198..590)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     promoter        complement(618..636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(619..636)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(656..673)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    662..773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aggaattcat ggctgccatc cggaagaaac tggtgattgt tggtgatgga gcctgtggaa
       61 agacatgctt gctcatagtc ttcagcaagg accagttccc agaggtgtat gtgcccacag
      121 tgtttgagaa ctatgtggca gatatcgagg tggatggaaa gcaggtagag ttggctttgt
      181 gggacacagc tgggctggaa gattatgatc gcctgaggcc cctctcctac ccagataccg
      241 atgttatact gatgtgtttt tccatcgaca gccctgatag tttagaaaac atcccagaaa
      301 agtggacccc agaagtcaag catttctgtc ccaacgtgcc catcatcctg gttgggaata
      361 agaaggatct tcggaatgat gagcacacaa ggcgggagct agccaagatg aagcaggagc
      421 cggtgaaacc tgaagaaggc agagatatgg caaacaggat tggcgctttt gggtacatgg
      481 agtgttcagc aaagaccaaa gatggagtga gagaggtttt tgaaatggct acgagagctg
      541 ctctgcaagc tagacgtggg aagaaaaaat ctggttgcct tgtcttgtga ctcgagcatg
      601 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
      661 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      721 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      781 ctgagtaggt gtcattctat tctggggggt
//
