LOCUS       sequence-112250-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc
       61 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
      121 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
      181 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
      241 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
      301 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
      361 cgaacagctg cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg
      421 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
      481 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
      541 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
      601 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc
      661 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga
      721 ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca
      781 gctt
//
