LOCUS       sequence-112251-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHK
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        133..320
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             321..983
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 cataattccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
       61 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      121 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      181 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      241 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      301 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      361 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      421 tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta
      481 acgtccacac aggtataggg tttctggacc atatgataca tgctctggcc aagcattccg
      541 gctggtcgct aatcgttgag tgcattggtg acttacacat agacgaccat cacaccactg
      601 aagactgcgg gattgctctc ggtcaagctt ttaaagaggc cctaggggcc gtgcgtggag
      661 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      721 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      781 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
      841 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
      901 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
      961 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgata
//
