LOCUS       sequence-112291-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of Tiam DH/PH-tgRFPt-Nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: mTiam1(64-437)-tgRFPt-SSPB WT
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        76..388
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     307..327
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     404..420
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     405..421
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(463..496)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
       61 gtaccgcgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacag
      121 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
      181 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
      241 tacattttac atgataggct tggatttcta taagagatac aaatactaaa ttattatttt
      301 aaaaaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta
      361 taaatatccc ttggagaaaa gccttgttaa cgcgcggtga ccctcgaggt cgacggtatc
      421 gataagctcg cttcacgaga ttccagcagg tcgagggacc taataacttc gtatagcata
      481 cattatacga agttatatta agggttccaa gcttaagcgg ccgcgtggat aaccgtatta
      541 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
      601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggc
//
