LOCUS       sequence-112295-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of murine Flag-Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt1
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     358..375
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tccccgtggt tcggggggcc tagacgtttt ttaacctcga ctaaacacat gtaaagcatg
       61 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
      121 ccttgttgaa tacgcttgag gtgagccatt tgactctttc cacaactatc caactcacaa
      181 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
      241 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
      301 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
      361 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
      421 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
      481 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
//
