LOCUS       sequence-112298-         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of murine Flag-Akt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt2
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     354..371
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gtggttcggg gggcctagac gttttttaac ctcgactaaa cacatgctaa agcatgtgca
       61 ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt
      121 gttgaatacg cttgaggtga gccatttgac tctttccaca actatccaac tcacaacgtg
      181 gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag
      241 gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg
      301 tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc
      361 tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg
      421 ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca
      481 ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggagg gagaggggcg
      541 ggatcctcac tctc
//
