LOCUS       sequence-112299-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with point mutation from Trp to Ala at position 80. 
            This mutation confers resistance to the MK-2206 drug.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt1W80A
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..158
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
       61 ctactagagg atctatttcc ggtgaattca tggactacaa agaccatgac ggtgattata
      121 aagatcatga catcgattac aaggatgacg atgacaagct tatgaacgac gtagccattg
      181 tgaaggaggg ctggctgcac aaacgagggg aatatattaa aacctggcgg ccacgctact
      241 tcctcctcaa gaacgatggc acctttattg gctacaagga acggcctcag gatgtggatc
      301 agcgagagtc cccactcaac aacttctcag tggcacaatg ccagctgatg aagacagagc
      361 ggccaaggcc caacaccttt atcatccgct gcctgcaggc caccacagtc attgagcgca
      421 ccttccatgt ggaaacgcct gaggagcggg aagaatgggc caccgccatt cagactgtgg
      481 cagatggact caagaggcag gaagaagaga cgatggactt ccgatcaggc tcacccagtg
      541 acaactcagg ggctgaagag atggaggtgt ccctggccaa gcccaagcac cgtgtgacca
      601 tgaacgagtt tgagtacctg aagctactgg gcaagggcac ctttgggaag gtgattctgg
      661 tgaaagagaa ggccacaggc cgctactatg ccatgaagat cctcaagaag gaggtcatcg
      721 tcgc
//
