LOCUS       sequence-112302-         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt2 with point mutation from Trp to Ala at position 80. 
            This mutation confers resistance to MK-2206 drug..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt2W80A
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             109..174
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgactctac tagaggatct atttccggtg aattcatgga ctacaaagac
      121 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagcttatg
      181 aatgaggtat ctgtcatcaa agaaggctgg ctccacaaac gtggtgaata catcaagacc
      241 tggaggccac ggtacttcct tctgaagagt gatggatctt tcattgggta taaggagagg
      301 cccgaggccc ctgaccagac cttacccccc ctgaacaatt tctctgtagc agaatgccag
      361 ctgatgaaga ctgagaggcc acgacccaac acctttgtca tacgctgcct gcaggccacc
      421 acagtcatcg agaggacctt ccatgtagac tctccagatg agagggaaga gtggatgcgg
//
