LOCUS       sequence-112303-         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt2 with point mutation from Trp to Ala at position 80. 
            This mutation confers resistance to MK-2206 drug..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt2W80A
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     359..376
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gtccccgtgg ttcggggggc ctagacgttt tttaacctcg actaaacaca tgtaaagcat
       61 gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc atccttcagc
      121 cccttgttga atacgcttga ggtgagccat ttgactcttt ccacaactat ccaactcaca
      181 acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg
      241 cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt
      301 gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc
      361 attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt
      421 ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac
      481 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggag
//
