LOCUS       sequence-112315-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for tandem expression of two sgRNAs from two independent U6 
            promoters. Cas9 is expressed by Cbh promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: pX333
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct
       61 gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt
      121 ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga
      181 aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct
      241 ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt
      301 cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt
      361 gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct
      421 tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt
      481 ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga
      541 acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg
      601 cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca
      661 gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca
      721 gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct
      781 tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc
      841 cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc
//
