LOCUS       sequence-112335-         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression vector with Gentamycin selection, based on 
            pBAD33.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Jimenez N, Lacasta A, Vilches S, Reyes M, Vazquez J, Aquillini E, 
            Merino S, Regue M, Tomas JM
  TITLE     Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide 
            core biosynthesis.
  JOURNAL   J Bacteriol. 2009 Apr;191(7):2228-36. doi: 10.1128/JB.01395-08. Epub
            2009 Jan 16.
  PUBMED    19151135
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01395-08"; journalName: "J Bacteriol"; date: "2009-04"; 
            volume: "191"; issue: "7"; pages: "2228-36"
COMMENT     Sequence Label: pBAD33-Gm
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    44..100
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(133..150)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      303..389
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      481..508
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcactctct actgtttctc catacccgtt tttttgggct agcgaattcg agctcggtac
       61 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga
      121 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
      181 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
      241 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
      301 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      361 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
      421 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
      481 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
      541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      601 tattg
//
