LOCUS       sequence-112337-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression vector with Gentamycin selection, based on 
            pBAD33.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Jimenez N, Lacasta A, Vilches S, Reyes M, Vazquez J, Aquillini E, 
            Merino S, Regue M, Tomas JM
  TITLE     Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide 
            core biosynthesis.
  JOURNAL   J Bacteriol. 2009 Apr;191(7):2228-36. doi: 10.1128/JB.01395-08. Epub
            2009 Jan 16.
  PUBMED    19151135
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01395-08"; journalName: "J Bacteriol"; date: "2009-04"; 
            volume: "191"; issue: "7"; pages: "2228-36"
COMMENT     Sequence Label: pBAD33-Gm
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(76..360)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(134..153)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgctag
       61 cccaaaaaaa cgggtatgga gaaacagtag agagttgcga taaaaagcgt caggtaggat
      121 ccgctaatct tatggataaa aatgctatgg catagcaaag tgtgacgccg tgcaaataat
      181 caatgtggac ttttctgccg tgattataga cacttttgtt acgcgttttt gtcatggctt
      241 tggtcccgct ttgttacaga atgcttttaa taagcggggt taccggtttg gttagcgaga
      301 agagccagta aaagacgcag tgacggcaat gtctgatgca atatggacaa ttggtttctt
      361 ctctgaatgg cgggagtatg aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg
      421 gatactcgtt taatgcccat ctggtggcgg gtttaacgcc gattgaggcc aacggttatc
      481 tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat ctcaccattc
      541 gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ttgccgaccg ggtgatattt
      601 tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct cgcgaatggt
      661 atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt aactggccgt
      721 caata
//
