LOCUS       sequence-112339-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flavonoid biosensor. High copy plasmid that expresses GFP 
            proportional to flavonoid concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-ttgR
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..151)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      304..390
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      483..510
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
       61 cctttttacg gttcctggcc ttttgctggc cttttgctca tggctgtttt ggcggatgag
      121 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
      181 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      241 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      301 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      361 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      421 caacggcccg gaggggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      481 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactctgc tagcaagtaa
      541 ggccgaccac ccagcagtat ttacaaacaa ccatgaatgt aagtatattc cttagcaaca
      601 tttaacttta agaaggagat atacatatgc gtaaaggtga agaactgttc accggtgttg
      661 ttccgatcct ggttgaactg gacggtgacg ttaacggtca caaattctct gttcgtggtg
      721 aaggtgaagg tgacgctacc aacggtaaac tgaccctgaa attcatctgc accac
//
