LOCUS       sequence-112343-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            drosophila vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAWG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(291..315)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtcatccacc ttagccatct cattactaaa gatctcctgc aggtagcaga tccgattctt
       61 tctgcgggta tatctgcgcc gtgctgttct tttgagccgc gtggcttcgg ccgtctcccc
      121 ggagtcgaac aggagggcgc caatgaggtt cttctttatg ctgtggcgat cggtattgcc
      181 cagaactttg aattttttgc tcggcacctt gtactcgtcc gtaatgacgg cccagccgac
      241 gctgtttgtg ccgatagcga gcccaatgga gtacttcttg tccattttgt agcctgcttt
      301 tttgtacaaa cttgtcgata tctggatccg gggtctctgg attagacgac tgctggctga
      361 tggagcggct ttgtgtcggg aggagtatcc acacagcaca agaactcaaa cggtagtgat
      421 atgaaaactg gtcccgcagc ccgttttata aagcccgctt agcgctaaaa ccttatcagc
      481 cgcaatgctc tccgctctca agtcgcgttc aaaactttta ccatatatgg gtatgaattg
      541 ttcgccgatt gggttttcat ttacggagaa tttcctccgc aactgggtgt tcgtgcttgt
      601 gtgtgtcttg tatatcgcca tgaagagtgt acttccatgg gtcctatcag ttgcggcatg
      661 tgattcaatt tgggggttac tcatattcga tacttcagct ggttatcttt atgctgtgat
      721 ggtcacggtg cttttacaca ttttttcccc aactactcat tgtatgccaa aacatacata
      781 tgtacatgtg tttatagtgg gtttattagt aatgt
//
