LOCUS       sequence-112347-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            drosophila vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAWG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaaaagcag gctacaaaat ggacaagaag tactccattg ggctcgctat cggcacaaac
       61 agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
      121 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
      181 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
      241 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
      301 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
      361 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
      421 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
      481 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
      541 aacccagaca acagcgatgt cgataaactc tttatccaac tggttcagac ttacaatcag
      601 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
      661 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
      721 aacggcctgt ttggtaatct tatcgccctg
//
