LOCUS       sequence-112350-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1(T17N)
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(48..575,576..578,579..626))
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(675..696)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1024..1043
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagtta caacagcagg
       61 cattttctct tcctcttctt cacgggaggc gggcagagga ctgctcggat cgcttcgtca
      121 aacactgtct tgaggcctcg ctgtgtgagc gccgagcact ccaggtattt tacagcacca
      181 atctccttag ccatggctag accctgcgga taggtgatgg gagtcagctt cttctccttc
      241 agtttctcga tcgtgtcttt atcatcccta agatcaagtt tagttcccac taggatgatg
      301 ggagtgttgg gacagtggtg ccgcacctca ggataccact ttgcacggac attttcaaat
      361 gatgcaggac tcacaaggga aaagcaaatt aagaacacat ctgtttgcgg ataggatagg
      421 gggcgtaatc tgtcataatc ttcttgtcca gctgtatccc ataagcccag attcaccggt
      481 tttccatcta ccataacatt ggcagaataa ttgtcaaaga cagtagggat atattctcca
      541 ggaaatgcat tggttgtgta actgatcagt aggcagtttt tacctacagc tccgtctccc
      601 accaccacac acttgatggc ctgcatgaat tccttgtaca gctcgtccat gccgagagtg
      661 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      721 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      781 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      841 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      901 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      961 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     1021 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagcc
//
