LOCUS       sequence-112353-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of nls-Cre from the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Beronja S, Livshits G, Williams S, Fuchs E
  TITLE     Rapid functional dissection of genetic networks via tissue-specific 
            transduction and RNAi in mouse embryos.
  JOURNAL   Nat Med. 2010 Jul . 16(7):821-7.
  PUBMED    20526348
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2010 Jul . 16(7):821-7."
COMMENT     Sequence Label: LV-Cre pLKO.1
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             111..131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagaa cgcgtcaccg actctagagg atctaccatg cccaagaaga
      121 agaggaaggt gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg
      181 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt
      241 ctgagcatac ctggaaaatg cttcagtccg tttgccggtc gtgggcggca tggtgcaagt
      301 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc
      361 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc
      421 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc
      481 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg
      541 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata
      601 tacgtaatct ggcatttctg gggattgctt ataaca
//
