LOCUS       sequence-112355-         522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of nls-Cre from the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Beronja S, Livshits G, Williams S, Fuchs E
  TITLE     Rapid functional dissection of genetic networks via tissue-specific 
            transduction and RNAi in mouse embryos.
  JOURNAL   Nat Med. 2010 Jul . 16(7):821-7.
  PUBMED    20526348
REFERENCE   2  (bases 1 to 522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2010 Jul . 16(7):821-7."
COMMENT     Sequence Label: LV-Cre pLKO.1
FEATURES             Location/Qualifiers
     source          1..522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        132..380
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     132..152
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     303..322
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
       61 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
      121 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      181 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      241 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      301 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      361 tgtggaaagg acgaaacacc ggtccgcagg tatgcacgcg tgaattctcg acctcgagac
      421 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      481 gggaaagaat agtagacata atagcgacag acatacaaac ta
//
