LOCUS       sequence-112357-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted version of the Ca2+ indicator GCaMP3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-Cyto-GCaMP3
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..36)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(43..483)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     599..618
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctagattac agggtcaggc gctccagggg gggcaggaat tccttcgctg tcatcatttg
       61 tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc
      121 atcaacctct tcatctgtta acttctctcc aaggtttgtc atcacgtggc gaagctctgc
      181 tgcactgatg tagccattgc catccttatc aaacacacgg aacgcttctc taatttcttc
      241 ttcactgtct gtgtctttca tttttcttgc catcattgtc aggaactcag ggaagtcgat
      301 tgtgccgtca ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg cttctgtggg
      361 gttctgcccc agagaccgca tcaccgtccc cagctccttg gttgttattg tcccatcccc
      421 gtccttgtca aatagggaga aagcctcttt aaattctgcg atctgctctt cagtcagttg
      481 gtcacgcgtg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      541 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      601 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggatgtagc cttcgggcat
      661 ggcggacttg aagaagtcgt gctgcttcat gtggtc
//
