LOCUS       sequence-112358-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endoplasmic reticulum (ER)-targeted version of a high-affinity 
            FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to 
            cytosolic face of ER membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3.1(+)-ER-BSCaM2
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(173..175,176..178,179..691)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(218..239)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(479..498)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgccaccatg gaccctgtgg tggtgctggg gctctgtctc tcctgtttgc ttctcctttc
      121 actctggaaa cagagctatg ggggagggaa gcttggtacc gagctcggat ccatggtgag
      181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      301 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      361 caccttcggc tacggcgtgc agtgcttcgc ccgctacccc gaccacatgc gccagcacga
      421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      601 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      661 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccga
//
