LOCUS       sequence-112360-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endoplasmic reticulum (ER)-targeted version of a high-affinity 
            FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to 
            cytosolic face of ER membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3.1(+)-ER-BSCaM2
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..722)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAH
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     403..422
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgctcgagtc acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
       61 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      121 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      181 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagtgggcc
      241 ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat gtagttgtac
      301 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      361 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      421 tccttgaaga agatggtacg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      481 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg
      541 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      601 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaacct gtggccgttt
      661 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      721 ctaccggtag cagccagacg gccaatcgca cgcaccgcat gt
//
