LOCUS       sequence-112362-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator 
            GCaMP3; targeted to cytosolic face of ER membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-ER-GCaMP3
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             195..212
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             216..248
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(252..260,261..275)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      282..291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             303..359
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     572..593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cccttgcagc ttccagttgt caggaagaga aggctcccat ggaccctgtg gtggtgctgg
      121 ggctctgtct ctcctgtttg cttctccttt cactctggaa acagagctat gggggagggg
      181 atcccatggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc
      241 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat
      301 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac
      361 tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttcaaga
      421 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
      481 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac
      541 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      601 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga
      661 gcaagggcga ggagctg
//
