LOCUS       sequence-112363-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator 
            GCaMP3; targeted to cytosolic face of ER membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-ER-GCaMP3
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..495)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     611..630
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cttacttcgc
       61 tgtcatcatt tgtacaaact cttcgtagtt tacctgacca tccccatcga tgtctgcttc
      121 cctgatcatt tcatcaacct cttcatctgt taacttctct ccaaggtttg tcatcacgtg
      181 gcgaagctct gctgcactga tgtagccatt gccatcctta tcaaacacac ggaacgcttc
      241 tctaatttct tcttcactgt ctgtgtcttt catttttctt gccatcattg tcaggaactc
      301 agggaagtcg attgtgccgt caccgtcggc atctacttca ttgatcatgt cctgcagctc
      361 tgcttctgtg gggttctgcc ccagagaccg catcaccgtc cccagctcct tggttgttat
      421 tgtcccatcc ccgtccttgt caaataggga gaaagcctct ttaaattctg cgatctgctc
      481 ttcagtcagt tggtcacgcg tgttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      541 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      601 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggatgta
      661 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
      721 gaagcactgc acgccgta
//
