LOCUS       sequence-112364-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring mTORC1 kinase activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-TORCAR
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(120..141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(381..400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      121 tggtcgagct ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg
      181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      241 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc
      301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      361 agcgtaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      481 acatcctggg gcacaagctg gagtacaacg ccatcagtga caacgtctat atcaccgccg
      541 acaagcagaa gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca
      601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      661 tgcccgacaa ccactacctg agcacccagt ccgccctgag c
//
