LOCUS       sequence-11237-e        2460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-FGFR1
FEATURES             Location/Qualifiers
     source          1..2460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(380..397)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtggagct ggaagtgcct cctcttctgg gctgtgctgg tcacagccac actctgcacc
       61 gctaggccgt ccccgacctt gcctgaacaa gcccagccct ggggagcccc tgtggaagtg
      121 gagtccttcc tggtccaccc cggtgacctg ctgcagcttc gctgtcggct gcgggacgat
      181 gtgcagagca tcaactggct gcgggacggg gtgcagctgg cggaaagcaa ccgcacccgc
      241 atcacagggg aggaggtgga ggtgcaggac tccgtgcccg cagactccgg cctctatgct
      301 tgcgtaacca gcagcccctc gggcagtgac accacctact tctccgtcaa tgtttcagat
      361 gctctcccct cctcggagga tgatgatgat gatgatgact cctcttcaga ggagaaagaa
      421 acagataaca ccaaaccaaa ccccgtagct ccatattgga catccccaga aaagatggaa
      481 aagaaattgc atgcagtgcc ggctgccaag acagtgaagt tcaaatgccc ttccagtggg
      541 accccaaacc ccacactgcg ctggttgaaa aatggcaaag aattcaaacc tgaccacaga
      601 attggaggct acaaggtccg ttatgccacc tggagcatca taatggactc tgtggtgccc
      661 tctgacaagg gcaactacac ctgcattgtg gagaatgagt acggcagcat caaccacaca
      721 taccagctgg atgtcgtgga gcggtcccct caccggccca tcctgcaagc agggttgccc
      781 gccaacaaaa cagtggccct gggtagcaac gtggagttca tgtgtaaggt gtacagtgac
      841 ccgcagccgc acatccagtg gctaaagcac atcgaggtga atgggagcaa gattggccca
      901 gacaacctgc cttatgtcca gatcttgaag actgctggag ttaataccac cgacaaagag
      961 atggaggtgc ttcacttaag aaatgtctcc tttgaggacg caggggagta tacgtgcttg
     1021 gcgggtaact ctatcggact ctcccatcac tctgcatggt tgaccgttct ggaagccctg
     1081 gaagagaggc cggcagtgat gacctcgccc ctgtacctgg agatcatcat ctattgcaca
     1141 ggggccttcc tcatctcctg catggtgggg tcggtcatcg tctacaagat gaagagtggt
     1201 accaagaaga gtgacttcca cagccagatg gctgtgcaca agctggccaa gagcatccct
     1261 ctgcgcagac aggtaacagt gtctgctgac tccagtgcat ccatgaactc tggggttctt
     1321 ctggttcggc catcacggct ctcctccagt gggactccca tgctagcagg ggtctctgag
     1381 tatgagcttc ccgaagaccc tcgctgggag ctgcctcggg acagactggt cttaggcaaa
     1441 cccctgggag agggctgctt tgggcaggtg gtgttggcag aggctatcgg gctggacaag
     1501 gacaaaccca accgtgtgac caaagtggct gtgaagatgt tgaagtcgga cgcaacagag
     1561 aaagacttgt cagacctgat ctcagaaatg gagatgatga agatgatcgg gaagcataag
     1621 aatatcatca acctgctggg ggcctgcacg caggatggtc ccttgtatgt catcgtggag
     1681 tatgcctcca agggcaacct gcgggagtac ctgcaggccc ggaggccccc agggctggaa
     1741 tactgctaca accccagcca caacccagag gagcagctct cctccaagga cctggtgtcc
     1801 tgcgcctacc aggtggcccg aggcatggag tatctggcct ccaagaagtg catacaccga
     1861 gacctggcag ccaggaatgt cctggtgaca gaggacaatg tgatgaagat agcagacttt
     1921 ggcctcgcac gggacattca ccacatcgac tactataaaa agacaaccaa cggccgactg
     1981 cctgtgaagt ggatggcacc cgaggcatta tttgaccgga tctacaccca ccagagtgat
     2041 gtgtggtctt tcggggtgct cctgtgggag atcttcactc tgggcggctc cccatacccc
     2101 ggtgtgcctg tggaggaact tttcaagctg ctgaaggagg gtcaccgcat ggacaagccc
     2161 agtaactgca ccaacgagct gtacatgatg atgcgggact gctggcatgc agtgccctca
     2221 cagagaccca ccttcaagca gctggtggaa gacctggacc gcatcgtggc cttgacctcc
     2281 aaccaggagt acctggacct gtccatgccc ctggaccagt actcccccag ctttcccgac
     2341 acccggagct ctacgtgctc ctcaggggag gattccgtct tctctcatga gccgctgccc
     2401 gaggagccct gcctgccccg acacccagcc cagcttgcca atggcggact caaacgccgc
//
