LOCUS       sequence-112370-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector to be used in the Dueber YTK system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK001
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      98..132
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(156..183)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(199..270)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
       61 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
      121 ggcgtttttt attggtctgg tcagagacgt ataaacgcag aaaggcccac ccgaaggtga
      181 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      241 gtctttcgac tgagcctttc gttttatttg atgcctggtc atttgtacag ttcatccata
      301 ccatgcgtga tgcccgctgc ggttacgaac tccagcagaa ccatatgatc gcgtttctcg
      361 ttcggatctt tagacagaac gctttgcgtg ctcagatagt gattgtctgg cagcagaaca
      421 ggaccatcac cgattggagt gttttgctgg tagtgatcag ccagctgcac gctgccatcc
      481 tccacgttgt ggcgaatttt aaaattcgct ttaatgccat ttttttgttt atcggcggtg
      541 atgtaaacat tgtggctgtt aaaattgtat tccagcttat ggcccaggat attgccgtcc
      601 tctttaaagt caatgccttt cagctcaatg cggtttacca gggtatcgcc ttcaaatttc
      661 acttccgcac gcgttttgta cgtgccgtca tccttaaagg aaatcgtgcg ttcctgcaca
      721 tagccttccg gcatggcgga cttgaagaag tcatgctgct tcatatggtc cggataacga
      781 gcaaagcact gaacaccata agtcagcgtc gttaccagag tcggccaagg aaccggcagt
      841 ttaccagtag tacagatgaa cttcagcgtc agtttaccat ta
//
