LOCUS       sequence-112372-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConLS as a Type 1 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK002
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..135
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(537..556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
       61 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      121 ccgggcgttt tttattggtc tggtctcacg ttcgtctgca attatcggcc agttcagtga
      181 gacggtattg cgacgaattg ccacgttgtc gcagtgtctg tactgtgggt taatgtctgt
      241 gatcgccgtc gtgagttcgt gtcatccgcg catgattcag atggtcctgg agatcgttgc
      301 ttgtaccggc tgtttgtggc tctaccatct agatgcgaat tcagggtgag accgactacg
      361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      901 agtatttggt atctgcgctc tgctgaagc
//
