LOCUS       sequence-112373-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConL1 as a Type 1 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK003
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      107..141
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(543..562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
       61 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      121 ttgccgccgg gcgtttttta ttggtctggt ctcacgttgt agctgtgttg acatctggtt
      181 tggtgagacg taactgcctt gatctgtcgg gtgtctgctt ctctcacagg cttaggtgga
      241 tctgctgtcc ccagtcagtg cggtcatcat gcagtcatcc gagcgtgtat tgcacacatt
      301 tgtttggcgc atgatgagcc gtgatgaccc cgttctagat gcgaattcag ggtgagaccg
      361 actacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacacta
//
