LOCUS       sequence-112382-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK009
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      107..141
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(164..830)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(271..294)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 tcacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
       61 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      121 ttgccgccgg gcgtttttta ttggtctggt ctcacataga tcttttgttt gtttatgtgt
      181 gtttattcga aactaagttc ttggtgtttt aaaactaaaa aaaagactaa ctataaaagt
      241 agaatttaag aagtttaaga aatagattta cagaattaca atcaatacct accgtcttta
      301 tatacttatt agtcaagtag gggaataatt tcagggaact ggtttcaacc ttttttttca
      361 gctttttcca aatcagagag agcagaaggt aatagaaggt gtaagaaaat gagatagata
      421 catgcgtggg tcaattgcct tgtgtcatca tttactccag gcaggttgca tcactccatt
      481 gaggttgtgc ccgttttttg cctgtttgtg cccctgttct ctgtagttgc gctaagagaa
      541 tggacctatg aactgatggt tggtgaagaa aacaatattt tggtgctggg attctttttt
      601 tttctggatg ccagcttaaa aagcgggctc cattatattt agtggatgcc aggaataaac
      661 tgttcaccca gacacctacg atgttatata ttctgtgtaa cccgccccct attttgggca
      721 tgtacgggtt acagcagaat taaaaggcta attttttgac taaataaagt taggaaaatc
      781 actactatta attatttacg tattctttga aatggcagta ttgataatga taaactcgaa
      841 ctgcgtttga gaccgactac ggttatccac agaatcaggg gataacgcag gaaagaacat
      901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      961 ccataggctc cgcccccctg acgagcat
//
