LOCUS       sequence-112387-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila Wingless protein under the tubulin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ching W, Hang HC, Nusse R
  TITLE     Lipid-independent secretion of a Drosophila Wnt protein.
  JOURNAL   J Biol Chem. 2008 Jun 20;283(25):17092-8. doi: 
            10.1074/jbc.M802059200. Epub 2008 Apr 22.
  PUBMED    18430724
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M802059200"; journalName: "J Biol Chem"; date: 
            "2008-06-20- 20"; volume: "283"; issue: "25"; pages: "17092-8"
COMMENT     Sequence Label: pTub-Wg
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    206..340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(230..249)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     284..303
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(612..632)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          762..827
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 agtgcgagtg aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata
       61 tgattgttga ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta
      121 gacgaaatta tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc
      181 aggtcgacct cgaggccttc tagtggatcc agacatgata agatacattg atgagtttgg
      241 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      301 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      361 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      421 caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atatttttcc
      481 ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca
      541 agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc
      601 ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta
      661 gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca
      721 ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt
      781 agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa
      841 atctctgtag gtagtttgtc
//
