LOCUS       sequence-112395-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Staphylococcus aureus Cas9 
            nuclease and GFP, plasmid optimized for expression in human 
            pluripotent stem cells and other mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  TITLE     Staphylococcus aureus Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSaCas9_GFP
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..85)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca ttgggccggg attctcctcc acgtcaccgc
       61 atgttagaag acttcctctg ccctctccgc cgccggacct gccctttttg ataatctgag
      121 ggtgcttttt gctcttcacc tcatacaggt ttcccagaat gtcggttgag tactttttga
      181 tactctgagt cttagaggcg attgttttga taattcgagg ggggcgctta tcattcatgt
      241 tttccagata ctctcggtaa gtgatgtcaa tcatattcac ttcaatgcgg ttcagcagat
      301 cattgttcac cccgatgacc ctatacagtt cgccattgat cttaatcagg tcgttgttgt
      361 aaaaggaggc gatgaactct gcctggttgc taatcttttt cagcttttta gcctcttcgt
      421 agcacttgct attcacttca tagtagttct cctttttgat gacatccaga ttcttgacag
      481 tcacaaattt atacacgccg ttgtccagat agacatcgaa tctgtatggc ttcagtgaca
      541 gcttgaccac cttgttgcga ctgttagggt aatcgtctgt gatgtccaga tgggcattca
      601 gcttgttccc atagtacttg atcttcttga tcacggggcc attatccttt ttgctatact
      661 tggtcaggta gttcccagtc tcttcatagt acttatacag tgggttcttc tcgtcgccgt
      721 actgctccat aatcagcttc agtttctgat atgtctgagg atcatggtgg tacatcagca
      781 gcttctcggg acttttgttg atcagctttt tcagcttgtc attatctttg tcgtacagtc
      841 cgttcagatt gttcacaatc agggtattcc ccttatcgtc ttttcttgta ctatacaggg
      901 tgtcattgat cagctctctg ttgggctttt tatccacccg gtgagagtac ttgtagtcct
      961 tgaaatcctt gatatgcttg atctggtgag gagtgatgaa aatctccttg tactcctgtt
     1021 ctgtctcgat ttcgggcata gattcggcct gcttctcttc gaacatctgg ttctccatca
     1081 ctttcttggc tttgtccagc tttt
//
