LOCUS       sequence-112404-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pTEF1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK013
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      105..139
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(175..588)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtctggtct cacatagatc ttttgtaatt aaaacttaga
      181 ttagattgct atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag
      241 aaaaatgaaa ctgaaacttg agaaattgat gaccgtttat taacttaaat atcaatggga
      301 ggtcatcgaa agagaaaaaa atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa
      361 aatttttatt gcctttttcg acgaagaaaa agaaacgagg cggtgtcttt tttcttttcc
      421 aaacctttag tacgggtaat taacgacacc ctagaggaag aaagagggga aatttagtat
      481 gctgtgcttg ggtgttttga agtggtacgg cgatgcgcgg agtccgagaa aatctggaag
      541 agtaaaaaag gagtagaaac attttgaagc tatggtgtgt gggggatcac ttgtggggga
      601 ttgggtgtga tgtaaggatt cgcggtcctc gaaaattaaa agtccaacgc gcctgttgct
      661 tcctatgtga tatgtattat atgtaatatg cataaatata tctactgcat tgtattttga
      721 acgtacaaag tatgcattgt ttatacgcta ttatcagcca aagttgggtg gtcgctttct
      781 gttgtatgac tattgatgtc taggctgtca ataatttcgt tttgagcctc catgtctctg
      841 aagaactccc tgttgg
//
