LOCUS       sequence-112406-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pTEF2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK014
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      105..139
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtctggtct cacatagatc tgtttagtta attatagttc
      181 gttgaccgta tattctaaaa acaagtactc cttaaaaaaa aaccttgaag ggaataaaca
      241 agtagaatag atagagagaa aaatagaaaa tgcaagagaa tttatatatt agaaagagag
      301 aaagaaaaat ggaaaaaaaa aaataggaaa agccagaaat agcactagaa ggagcgacac
      361 cagaaaagaa ggtgatggaa ccaatttagc tatatatagt taactaccgg ctcgatcatc
      421 tctgcctcca gcatagtcga agaagaattt tttttttctt gaggcttctg tcagcaactc
      481 gtattttttc tttctttttt ggtgagccta aaaagttccc acgttctctt gtacgacgcc
      541 gtcacaaaca accttatggg taatttgtcg cggtctgggt gtataaatgt gtgggtgcaa
      601 catgaatgta cggaggtagt ttgctgattg gcggtctata gataccttgg ttatggcgcc
      661 ctcacagccg gcaggggaag cgcctacgct tgacatctac tatatgtaag tatacggccc
      721 catatatata tatatatata tacattaaac attattggta aatacaccag caaccgcatg
      781 attgatgtta tggtggaata taggtagcta aaaaaactct acataacaaa gt
//
