LOCUS       sequence-112407-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pHHF1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK015
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      105..139
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtctggtct cacatagatc tattttacta tattatattt
      181 gttgcttgtt tttgtttgtt gctttagtac tatagagtac aataatgcga cggaaaccat
      241 catatagaaa aaatatctcg gtatttatag gaaaaagaat tagacctttt ccacaaccaa
      301 tttatccatc aaattggtct ttacccaatg aatggggaag ggggggtggc aatttaccac
      361 cgtattcgcg ggcatttgct aaagtaaaca acttcggttt ttaccactaa ccattatggg
      421 gagaagcgct cggaacagtt ttactatgtg aagatgcgaa gttttcagaa cgcggtttcc
      481 aaattcggcg gggagataca aaaaagattt ttgctctcgt tctcacattt tcgcattgtc
      541 ccatacatta tcgttctcac aatttctcac atttccttgc tctgcacctt tgcgatcctg
      601 gccgtaatat ctctccttga cttttagcgt ggaagataac gaaatgcccg ggcgattttt
      661 ctttttagta ccctccacgg ctccttgttg aaatacatat ataaaagact gtgtattctt
      721 cgggatacat ctctttcctc aaccttttat attctttctt tctagttaat aagaaaaaca
      781 tctaacataa atatataaac gcaaacaatg gccagaacaa agcaaacagc aagaaagtcc
      841 actggtggta aggccccaag acgtttgaga ccgactacgg ttatccacag aatcagggga
      901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      961 cgcgttgctg g
//
