LOCUS       sequence-112409-        3355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of ABKAR biosensor targeted to the lysozome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: LAMP1-ABKAR
FEATURES             Location/Qualifiers
     source          1..3355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(98..181,182..1348)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFAYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTPHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             join(1373..1375,1376..1378,1379..2089)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2612..3346
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 agcnganctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgcggc cgccaccatg gcggcgcccg gcagcgcccg
      121 gcgacccctg ctgctgctac tgctgttgct gctgctcggc ctcatgcatt gtgcgtcagc
      181 agcaatgttt atggtgaaaa atggcaacgg gaccgcgtgc ataatggcca acttctctgc
      241 tgccttctca gtgaactacg acaccaagag tggccctaag aacatgacct ttgacctgcc
      301 atcagatgcc acagtggtgc tcaaccgcag ctcctgtgga aaagagaaca cttctgaccc
      361 cagtctcgtg attgcttttg gaagaggaca tacactcact ctcaatttca cgagaaatgc
      421 aacacgttac agcgtccagc tcatgagttt tgcttataac ttgtcagaca cacacctttt
      481 ccccaatgcg agctccaaag aaatcaagac tgtggaatct ataactgaca tcagggcaga
      541 tatagataaa aaatacagat gtgttagtgg cacccaggtc cacatgaaca acgtgaccgt
      601 aacgccccat gatgccacca tccaggcgta cctttccaac agcagcttca gcaggggaga
      661 gacacgctgt gaacaagaca ggccttcccc aaccacagcg ccccctgcgc cacccagccc
      721 ctcgccctca cccgtgccca agagcccctc tgtggacaag tacaacgtga gcggcaccaa
      781 cgggacctgc ctgctggcca gcatggggct gcagctgaac ctcacctatg agaggaagga
      841 caacacgacg gtgacaaggc ttctcaacat caaccccaac aagacctcgg ccagcgggag
      901 ctgcggcgcc cacctggtga ctctggagct gcacagcgag ggcaccaccg tcctgctctt
      961 ccagttcggg atgaatgcaa gttctagccg gtttttccta caaggaatcc agttgaatac
     1021 aattcttcct gacgccagag accctgcctt taaagctgcc aacggctccc tgcgagcgct
     1081 gcaggccaca gtcggcaatt cctacaagtg caacgcggag gagcacgtcc gtgtcacgaa
     1141 ggcgttttca gtcaatatat tcaaagtgtg ggtccaggct ttcaaggtgg aaggtggcca
     1201 gtttggctct gtggaggagt gtctgctgga cgagaacagc atgctgatcc ccatcgctgt
     1261 gggtggtgcc ctggcggggc tggtcctcat cgtcctcatc gcctacctcg tcggcaggaa
     1321 gaggagtcac gcaggctacc agactatcgg tggtggcggt ggtgcggatc ccatggtgag
     1381 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1441 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1501 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1561 caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1681 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1801 gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga acggcatcaa
     1861 ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1981 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2041 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc gcatgcataa
     2101 gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg gtcatttgta ccacgggtca
     2161 aattcccatc cgagatttgt cagctgatat ttcacaagtg cttaaggaaa aacgatccat
     2221 aaagaaagtt tggacatttg gtagaaaccc agcctgtgac tatcatttag gaaacatttc
     2281 aagactgtca aataagcatt tccaaatact actaggagaa gacggtaacc ttttattgaa
     2341 tgacatttcc actaatggga cctggttaaa tgggcaaaaa gtcgagaaga acagcaatca
     2401 gttactgtct caaggtgatg aaataaccgt tggtgtaggc gtggaatcag atattttatc
     2461 tctggtcatt ttcataaacg acaaatttaa gcagtgcctc gagcagaaca aagttgatcg
     2521 ctctgcaggt aagccaggca gcggcgaggg cagcaccaag atgcgtcgtg tcgccaccct
     2581 ggtggacctc ggcaccggcg gcagcgagct catgggcggc gtgcagctcg ccgaccacta
     2641 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2701 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2761 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gaggtaccgg
     2821 tggatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2881 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2941 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     3001 gcccaccctc gtgaccaccc tgggctacgg ccttcagtgc ttcgcccgct accccgacca
     3061 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3121 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3181 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3241 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     3301 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgagtaag aattc
//
