LOCUS       sequence-112413-        3338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of chicken aconitase GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Sagar, Prols F, Wiegreffe C, Scaal M
  TITLE     Communication between distant epithelial cells by filopodia-like 
            protrusions during embryonic development.
  JOURNAL   Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. 
            Epub 2015 Jan 23.
  PUBMED    25617437
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115964"; journalName: "Development"; date: "2015-02-15-
            15"; volume: "142"; issue: "4"; pages: "665-71"
COMMENT     Sequence Label: pCAG aconitase GFP
FEATURES             Location/Qualifiers
     source          1..3338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2375..2427
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2431..2440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2437..2439,2440..2442,2443..3156)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2437..2439,2440..2442,2443..3153)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2743..2762)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3090..3111
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3246..3265)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 atcatcgata tggcgccgta ctgcgtctgg ctggctcggc tgcggcatgc cctgaatggt
       61 gggatacggc gctaccatgt tgcatctgtt ctatgccaga gggccaaggt ggccatgagc
      121 cactttgagc ctaatgaata cataaactat gaaaagttgg agaagaacat caacatcgtc
      181 cgtaagaggc ttgaccgtcc cctgacctta tctgagaaga ttgtatatgg acacctggat
      241 gatccagcaa aacaggagat tgagagaggc aagacctatt tgcggctgcg gcctgaccgt
      301 gtggccatgc aggatgccac tgctcagatg gcaatgctac agttcatcag cagcgggctg
      361 ccgaaagtgg ccgtgccttc caccatccac tgtgatcacc tgatagaagc tcagttaggt
      421 ggtgaaaagg atcttcgaag agccaaggac ataaaccagg aggtgtacaa ctttctatcg
      481 acggctggtg ccaagtatgg agtgggattc tggaaacctg gatcaggaat cattcaccag
      541 atcattctgg agaactactc gtaccctggg gttatgttga ttggcacaga ctcacacacc
      601 cccaacggag gtggcttggg aggaatctgc attggagtgg gtggagctga tgctgtagat
      661 gtcatggcag gaatcccttg ggagctcaaa tgcccaaagg ttattggtgt aaaactaact
      721 gggaagcttt ctgggtggag ttctcctaaa gatgtgatcc tgaaagtggc tggcatcctc
      781 actgttaagg gtggtacagg tgccatcatc gaataccatg ggcctggtgt ggattcgatc
      841 tcttgcactg gaatggcaac aatctgtaac atgggagctg aaattggagc taccacctca
      901 gtcttccctt acaacacccg gatgaagaaa tacctgggca agactgggcg agctgacata
      961 gctgcactgg cagatgagtt ccagcaatac ttggtgccag atgctggttg tcagtacgac
     1021 caggtgatag aaatcaacct cagtgagctg aagccacata tcaatggacc tttcacacca
     1081 gacctggcgc accctgtgtc agatgttggt gctgtggcag aaaaggaggg ctggcctgtt
     1141 gatatcagag ttggcctgat tggcagctgc accaactcca gctatgagga catgggacgc
     1201 tctgcagcag tggcaaagca ggcactggcg catgggttga agtgcaaatc aaagttcaca
     1261 atcacaccag gctcagagca aatccgtgcc accattgaaa gggacggtta cgcgcaaatc
     1321 ctgcgagacg ttggagggct gattcttgcc aatgcttgtg ggccatgcat tggccagtgg
     1381 gacaggaagg acataaagaa aggagagaaa aacacaatag ttacgtctta caaccggaat
     1441 ttcacaggtc gcaatgatgc caacccagag actcacgcgt ttgtgacctc cccagagatt
     1501 gtcacagccc tgtccattgc tggtactcta aaatttaacc ctgagacaga tttcctgaca
     1561 ggagcagatg ggaagaagtt taaactagaa gcacctgatg cagatgagct gcctaggctg
     1621 gattttgacc caggccagga cacgtatcag taccctccca aggatggcag tgggcagcat
     1681 gtggatgtga gccccaccag ccagcgcctc cagcttcttg agccctttga taagtgggat
     1741 ggcaaggatc tagaagacat gctgatcctc atcaaggtaa aagggaaatg caccactgat
     1801 catatttctg ctgctggacc atggctcaaa ttccgtggcc atttggacaa catctccaac
     1861 aacctgctca ttggcgctat caacattgaa aatggcaaag ccaactctgt gaggaatgca
     1921 ctaacccagg agtttgggcc tgtcccagac acagcccgtt actataagaa aatgggtgtc
     1981 aaatgggcag ttattgggga tgaaaactat ggtgagggat caagccgaga gcacgcagcc
     2041 ttggagccac gtcacttagg gggcagggtg atcatcacca agagctttgc caggatccac
     2101 gaaaccaacc tgaagaagca aggtctgctg cctcttactt ttgcggatcc agcagactac
     2161 aacaagattc atcctgtgga caagctgagc atcgtggggc tggcagactt tgctcctgga
     2221 aagcctctga aatgcatcat caagcatccc aacgggagcc aagaaacaat catgttgaac
     2281 cacaccttca acgagtcgca gatcgagtgg ttccaggctg gcagtgccct gaacagaatg
     2341 aaggagctgc agcagaaatc aagcgcggcc gcacctcgag ctcaagcttc gaattctgca
     2401 gtcgacggta ccgcgggccc gggatccatc gccaccatgg tgagcaaggg cgaggagctg
     2461 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2521 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2581 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2641 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2701 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2761 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2821 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2881 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2941 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3001 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3061 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3121 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcaattc actcctcagt
     3181 gcaggctgcc tatcagaagt ggtggctggt gtggcccatg ccctggctca caaataccac
     3241 tgagatcttt ttccctctgc caaaattatg gggacatcat gaagcccctt gagcatctga
     3301 cttctggcta ataaaggaaa tttattttca ttgcatag
//
