LOCUS       sequence-112416-        2856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of chicken tubulin GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Sagar, Prols F, Wiegreffe C, Scaal M
  TITLE     Communication between distant epithelial cells by filopodia-like 
            protrusions during embryonic development.
  JOURNAL   Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. 
            Epub 2015 Jan 23.
  PUBMED    25617437
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115964"; journalName: "Development"; date: "2015-02-15-
            15"; volume: "142"; issue: "4"; pages: "665-71"
COMMENT     Sequence Label: pCAG tubulin GFP
FEATURES             Location/Qualifiers
     source          1..2856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1510..1562
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1566..1575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1572..1574,1575..1577,1578..2291)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1572..1574,1575..1577,1578..2288)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1617..1638)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1878..1897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2225..2246
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2383..2402)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(2503..2522)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ggcgggattc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
       61 tgccttcttc tttttcctac agctcctggg caacgtgctg gttaatkgtg ctgtctcatc
      121 attttggcaa agaattgctc gagggtcgga tcgacggtat cgatatgcgt gagatcgtgc
      181 acatccaggc cgggcagtgc ggcaaccaga tcggcgccaa gttctgggag gtcatcagcg
      241 atgagcacgg catcgacccc accggcagct accacggcga cagcgacctg cagctggaga
      301 ggatcaacgt gtactacaat gaagctgccg gtaacaagta cgtcccccgt gccatcctgg
      361 ttgacctgga gcccggcacg atggactcgg tgcgctccgg cccctttgga cagatcttcc
      421 gacccgacaa ctttgtcttt ggtcagagcg gggccggcaa caactgggcc aaggggcact
      481 acacggaggg cgctgagctg gtggactctg tgctggatgt ggtgaggaag gagtcggaaa
      541 gctgcgactg cctgcagggc ttccagttga cccactcgct gggcggcggc accggctcgg
      601 ggatggacac cctcctcatc agcaagatcc gcgaggagta ccccgaccgc atcatgaaca
      661 cgttcagcgt catgccctcc cccaaggtgt cggacacggt ggtggagccc tacaatgcca
      721 ccctttctgt gcaccagctg gtggagaaca cggacgagac ctactgcatc gacaacgagg
      781 ccctgtacga catctgcttc cgcaccctga agctcaccac tcccacgtac ggggacctca
      841 accacctggt gtcggccacc atgagcggcg tgaccacctg ccttcgcttc cccggccagc
      901 tgaacgccga cctgcgcaag ctggcggtca acatggtgcc tttcccccgg ctgcacttct
      961 tcatgccggg cttcgccccg ctgacgagcc gcggcagcca gcagtaccga gccctgacgg
     1021 tgcccgagct gacgcagcag atgttcgact ccaagaacat gatggccgcc tgcgaccccc
     1081 gccacggccg ctacctgacg gtggctgcca tcttccgagg ccgcatgtcc atgaaggagg
     1141 tggacgagca gatgctgaac gtgcagaaca agaacagcag ctactttgtg gagtggatcc
     1201 ccaacaacgt gaagacggcc gtctgcgaca tccccccgcg tggcctcaag atgtccgcca
     1261 ccttcatcgg caacagcacg gccatccagg agctcttcaa gaggatctcg gagcagttca
     1321 cggccatgtt ccggcgcaag gccttcttgc actggtacac cggcgagggc atggatgaga
     1381 tggagttcac cgaggcggag agcaacatga acgacctggt ctcagagtac cagcaatacc
     1441 aggatgccac cgctgatgag cagggtgaat ttgaagagga aggagaggag gatgaggcag
     1501 cggccgcacc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     1561 ccatcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1621 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1681 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1741 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1801 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1861 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1921 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1981 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2041 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     2101 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2161 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2221 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2281 tgtacaagta aagcggccgc aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     2341 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     2401 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaagggaaat
     2461 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     2521 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     2581 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     2641 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     2701 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     2761 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc ccttcttctc
     2821 ttatggagat ccctcgaccc tgcaccgtcg accagc
//
