LOCUS       sequence-112419-        2508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of chicken Fzd7 GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Sagar, Prols F, Wiegreffe C, Scaal M
  TITLE     Communication between distant epithelial cells by filopodia-like 
            protrusions during embryonic development.
  JOURNAL   Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. 
            Epub 2015 Jan 23.
  PUBMED    25617437
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115964"; journalName: "Development"; date: "2015-02-15-
            15"; volume: "142"; issue: "4"; pages: "665-71"
COMMENT     Sequence Label: pCAG Fzd7 GFP
FEATURES             Location/Qualifiers
     source          1..2508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1709..1761
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1765..1774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1771..1773,1774..1776,1777..2490)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1771..1773,1774..1776,1777..2487)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1816..1837)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2077..2096)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2424..2445
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcgatatgc ggcccgcggc gggagaagcg ggcgccgggc tccgctggct gggcctggcc
       61 gccctgctgg ccgccctgct gggcacaccg tgcgcggcgg cgcatcagga cgataaggcc
      121 atctccgtgc cggaccacgg cttctgccag cccatctcca tcccgctctg cacggatatc
      181 gcctacaacc agaccatcct gcccaacctg ctgggccaca ccaaccagga ggacgcgggc
      241 ctggaggtgc accagttcta cccactggtc aaggtgcagt gctcggccga gctcaagttc
      301 ttcctctgct ccatgtacgc accggtttgc accgtattgg agcaggccat cccaccctgc
      361 cgctccctat gtgagcgggc tcgccagggt tgcgaggcgc tcatgaacaa gtttggcttc
      421 cagtggccgg agcggctccg ctgcgagaac tttcctgtgc acggtgcggg cgagatctgc
      481 gtggggcaga acacgtcgga tgctccgtca gggcccggcg gcgcgggggg tcggggagcc
      541 acggcgcagc ccacagccgg ctacctgccc gacctcctca ccccaccgca gcccgctgcc
      601 gggttctcct tctcttgccc tcggcagctc aaggtgcccc cctacttggg ctaccgtttc
      661 ctgggggagc gggactgtgg agccccctgt gagccgggcc ggcccaacgg gctcatgtac
      721 ttcaaggagg cagaggtacg ttttgcccgg ctctgggtgg gtgtgtggtc cgtgctctgc
      781 tgcgcgtcca ccctctttac agtgcttacc tacctggtgg acatgcgccg cttcagctac
      841 ccagagcgac ccatcatctt cctctcaggc tgctacttca tggtggcggt ggcttacgca
      901 gcgggtttcc tgctggagga gcgggtggtg tgcctggagc gcttctctga ggacggctac
      961 cgtaccgtgg cccaaggcac caagaaagaa ggctgcacca tcctcttcat gatcctctac
     1021 ttcttcggta tggccagctc catctggtgg gtcatactgt cccttacctg gtttctggca
     1081 gctggcatga aatggggcca cgaggccatc gaggccaact cccagtactt ccacctggct
     1141 gcctgggctg tgcccgctgt caaaaccatc accatcctag ctatggggca ggtggatgga
     1201 gatgtgctca gtggggtgtg ctatgtgggc atctacagcg tggactcgct gcggggcttt
     1261 gtgctagcac cgctcttcgt gtacctcttc attggcacct ccttcctgct ggctggcttt
     1321 gtgtccttgt ttcgcatacg caccatcatg aaacatgatg gcaccaagac agagaagctg
     1381 gagaagctga tggtgcgcat cggggtcttc agtgtcctct acacagtccc tgccaccatt
     1441 gtcctggcat gctacttcta tgagcaggcc ttccgtagca cctgggagaa gacgtggctc
     1501 ctccagacat gcaaaaccta cgctgtgccc tgccctagcc actttgcccc catgagcccg
     1561 gacttcacag tcttcatgat caagtacctc atgactatga tcgttggcat cacgacgggc
     1621 ttctggatct ggtctggtaa aaccctacag tcctggcggc gcttctacca cagactgagc
     1681 accggcagca agggcgagac ggcggtatct cgagctcaag cttcgaattc tgcagtcgac
     1741 ggtaccgcgg gcccgggatc catcgccacc atggtgagca agggcgagga gctgttcacc
     1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2221 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2461 actctcggca tggacgagct gtacaagtaa agcggccgcg actctaga
//
