LOCUS       sequence-112422-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pRAD27 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK025
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..138
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
       61 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      121 ccgccgggcg ttttttattg gtctggtctc acatagatct attttttctt ccggtgtccg
      181 tttcctattt ctttccaatg tatgctgtca acgcttttca tcgatgtata taaggaagaa
      241 tacctcatta taatacatca aaaccttcat ttgcgcgatg ttacgcgtcg cgtttcctat
      301 aaaaaataca taagctagtg aataaaagct ttaaacgcgt tagggggatc tgcaattaaa
      361 gtaaataaat gaaataaatg aaatagatga aaaactggtt acactaatta taacgttaat
      421 aaaactagct gataatcaaa atattgccat agctaagcca atagtacagc gttggctttc
      481 tatcataact taccagccgg gtgcccaatc gggtatctgt caccgaaagt cactgttaac
      541 gaggtgtgat tcagccgggt acttgtctag catttttttt cgcctgtttt tttcgtgaaa
      601 aacgaaaagt ggcaagcaaa cttatatcac tttctacttc tttccctgtt ctttttgttt
      661 attggcaatg gccactacca catccccatt aacgaaagtc accaaagtgt gtcatttaat
      721 actacggtta gtgacagcat tttgtttagg tcttaactag agaggattat caactgctaa
      781 gccctttaca cgttatctgt gatcctgctg cactaggaaa cgtttcaaat caccatattt
      841 gcaatttcac aaggcgttt
//
