LOCUS       sequence-112423-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase 
            activity; targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-TORCAR
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cttactattt
       61 gtacaattca ttcataccct cggtaatacc agcagcagtc aaaaattcta acaagaccat
      121 gtggtctctc ttttcgtttg gatctttgaa taaggcagat tgataggata agtaatggtt
      181 gtctggtaac aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa
      241 ttgaacacca ccatcttcaa tgttgtgtct aattttgaag ttagctttga taccattctt
      301 ttgtttgtca gcagtgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc
      361 taaaatgtta ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt
      421 atcaccttca aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat
      481 agttctttct tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat
      541 atgatctggg tatctagcaa aacattgaac accataacct aaagtagtga ctaaggttgg
      601 ccatggaact ggcaatttac cagtagtaca gagtaatttt aaggtcaatt taccgtacgt
      661 agcatcacct tcaccttcac cggagacag
//
