LOCUS       sequence-112426-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK027
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..135
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
       61 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      121 ccgggcgttt tttattggtc tggtctcaca tagatctcgc tggatatgcc tagaaatgca
      181 gtatcgattt attttgagaa aatctgtcaa ctcgttactt ctttgatagg gaaatcttta
      241 gttaaatttg tttaaaatta agaaccaggt ctttagacta tgccaaaagg tattggtgct
      301 taaaggttgc cgtaaactct aactctatat atctctaagc caatgtttaa gttgatgcga
      361 tgtgcatgat cacttcacat ttttttcatg attttgtttt cttccttagc tacttttttc
      421 cgtcttggtc tgtgtaaaaa aaaaatcgct tcactgaaga aaaatataaa taaaaaaagg
      481 acatcagcaa gttcgtagtc aataaatgtg atgagtaatt tgcgataatc taccccaact
      541 caaagcattc aattcaaatg aaagaagagt tcgtgaattt taatctggct tacagattgg
      601 atcatagctt ctaatatatc ttccttttta ttttaaagtg attaaaatat gtggatgtct
      661 gcattaaggt gaaggcagac tatagtgaaa aatagtagca aaggcttcat ataatgaact
      721 acctagttgt attcaattgg aatctgtgaa cgcggcatta aaagaatatt tgagcttttt
      781 ttgattgtat taacctgaag ctatcacggt ttagactcat taaaagaaaa atatccggtt
      841 gtatcggata acaacaccgt ttgagaccga ctacggttat ccacagaatc aggggataac
      901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaaaggcc
//
