LOCUS       sequence-112429-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pMFA1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK028
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      105..139
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtctggtct cacatagatc tttctattcg atggctttgt
      181 acttattttg gttttatccg agaaacagtt acagatccct ctcattaatt catttctggc
      241 ggtttatata tgatgactct atttcatttt tttacttgat tttcagcaac cactgaatct
      301 gtatctttat ctctaccatt ttaggtttct taactgtttt ttctgattac tgggtttcat
      361 ttaacatgta aatttccttt ttgggtaatt acacactcgt ttgtaacaag aacaaagaat
      421 aaaggtatgg gccaaccgta gcagtaggtg ccttttttct gaacactgaa acagatcgtg
      481 aatgcatgta tatgcgtaca tagaaaataa aaagtagttg attacatagc ttatgccacg
      541 ttgcacacta tcctgatgtt ttaaagctcc tcggatataa cacatcctaa gcccataaat
      601 gcctctgtca acaccctttc gtacgcataa ttagtgttga acccagacgc aataaattct
      661 ttcctgtgct caagatgttt gatgaatggg tcattgtata ctgaaccttt caacgtaaac
      721 cttgggtgtg cttctctatc accgttaaac ggcgtgaacg gtacagcatc tttgagtctc
      781 gaattgtgta cgttcacatt tgaattgaaa tatggattgt ttttcagaac tctatttagt
      841 tcttccaatt tccttctagc gcgtttgaga ccgactacgg ttatccacag aatcagggga
      901 taacgcagga aagaac
//
