LOCUS       sequence-112430-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase 
            activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-TORCAR-NLS
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..39)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     437..456
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctagattat gcgtcttcca cctttctctt ctttttgggg aattctttgt acaattcatt
       61 cataccctcg gtgatgccgg cggcggtcag gaactccagc agcaccatgt ggtcccgctt
      121 ctcgttgggg tccttgaaca gggcgctctg gtagctcagg tagtggttat cgggcagcag
      181 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggccagct gcacgccgcc
      241 gtcctcgatg ttgtgccgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc
      301 ggtgatgtac acgttgtggc tgttgtagtt gtactccagc ttgtggccca ggatgttgct
      361 gtcctccttg aagtcgatgc ccttcagctc gatccggttc accagggtgt cgccctcgaa
      421 cttcacctcg gcccgggtct tgtagttgcc gtcgtccttg aagaagatgg tccgctcctg
      481 cacgtagccc tcgggcatgg cgctcttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      541 ccgggcgaag cactgcacgc cgtagcccag ggtggtcacc agggtgggcc agggcacggg
      601 cagcttgccg gtggtgcaca gcagcttcag ggtcagcttg ccgtaggtgg cgtcgccctc
      661 gccctcgccg ctcacgctga acttgtggcc gttcacgtcg ccgtccagct ccaccaggat
      721 gggcaccacg ccggtgaaca gc
//
