LOCUS       sequence-112436-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pCUP1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK031
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      105..139
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(218..452)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(662..681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtctggtct cacatagatc ttttatgtga tgattgattg
      181 attgattgta cagtttgttt ttcttaatat ctatttcgat gacttctata tgatattgca
      241 ctaacaagaa gatattataa tgcaattgat acaagacaag gagttatttg cttctctttt
      301 atatgattct gacaatccat attgcgttgg tagtcttttt tgctggaacg gttcagcgga
      361 aaagacgcat cgctcttttt gctcctagaa gaaatgccag caaaagaatc tcttgacagt
      421 gactgacagc aaaaatgtct ttttctaact agcaacaagg ctaagatcgt ttgagaccga
      481 ctacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      541 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagca
//
